Posted by admin | Posted in fly fishing | Posted on 05-03-2011
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Medium Crystal
Membrane Protein Structure Tools and Bicelle Crystallization
Membrane protein structure biology is one of the rapidly growing fields in biosciences. Lots of research has been done in the field of membrane crystallization. Though a vast number of proteins have been studied and the structure known, some of the protein targets are immune o traditional approach to membrane protein crystallization with detergents. This has given rise to the emergence of lipidic phase crystallization which includes lipidic cubic phase and bicelle crystallization methods.
These new methods help in crystallizing membrane proteins by long term structural stability and there by proper crystallization. As the only reliable way to assess the structure of any protein is crystallography, technology is rapidly growing to refine the crystallization process for immune membrane proteins.
Diffraction at high resolution when obtaining crystals of membrane proteins remains the great stumbling block in membrane protein structure determination. Crystallizing membrane proteins from a bicelle lipid/detergent mixture is a new method in protein crystallization. Protein structure determination with the help of bicelle is flexible and simple.
Bicelles are just disk-like micelles, formed by mixing phosphatidylcholine lipid such as Dimyristoyl-phosphatidylcholine (DMPC) and a detergent such as 3-[(3- cholamidopropyl) dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO). The bicelle discs can be described as patches of lipid bilayers with detergent molecules lining the apolar edges of each bilayer. Bicelles present a compromise between a rigid lipidic medium and an artificial detergent medium while offering beneficial aspects from both. Membrane proteins can be readily reconstituted into bicelles and are maintained in a native-like bilayer environment, which can be manipulated with almost the same ease as detergent solubilized membrane proteins, making it compatible with standard high-throughput screening.
There are many membrane protein structure tools are available in the market for the research of bicelle crystallization with which one can successfully crystallize membrane proteins and determine protein structure. Recently, a significant number of membrane proteins have been successfully crystallized using the bicelle method, including Bacteriorhodopsin, β2 adrenergic receptor/Fab, Voltage-Dependent Anion Channel and Xanthorhodopsin.
About the Author
MemMagic Bicelle Screen Kit is a bicelle crystallization tool, produced by MemX Biosciences, a leading producer of membrane protein crystallization tools. For more details, please visit http://www.memxbio.com .
Why won't my BlackBerry play crystal clear youtube videos?
I have a BlackBerry Curve 8310 (has 4.5 OS), which I got about 4 months ago, and I was told by the at&t sales representative that the phone could play videos on youtube. While it does play youtube videos, the video quality is very poor. The picture is all messed up and the sound quality has static, unlike the sound quality of mp3 music on my media player which sounds exceptional. Why is this?
the conversion for it to play on your blackberry pixelates the video. there isn't really anything you can do about that.
you can always download videos on your blackberry
this site www.mydatadetails.com converts them for you. just email the person running the site and request the video. they will convert it to the best quality.
check out the VIDEOS TO DOWNLOAD section on the right of the site and see what videos they have already converted. you will want the H.264 version bc its the best quality.
they also have a BLACKBERRY BASIC section that will explain the differences of each file. like .3pg, mpg4, h.263, h.264 and etc.
Medium Salt Crystal Lamp (SLM006)